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Difference between auto tuning and manual tuning of lc ms. LC-MS Calibration vs. There are two procedures that are used to make sure that an HPLC equipped with a mass spectral detector (MS) is reporting proper mass information and that the system is properly set to detect the analyte(s) of interest. These are calibration and tuning – two processes that are sometimes confused. Manual Tuning with a Long Dead Time This kind of situation is common with temperature loops because dead time is usually a significant factor. This approach is best used where a recorder is connected to the controller. Standard Spectra tune has ratio targets for it to achieve for the specific ions that it is looking for. This means that the Autotune has a minimum target but no maximum where as the Standard Spectra Tune has a minimum and a maximum for the ions it is looking for. Let’s look at the Tune Wizard for each tune and see what can be.
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